Cell Fractionation of Pseudomonas aeruginosa

Cell Fractionation of Pseudomonas aeruginosa

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Pseudomonas aeruginosa is a Gram negative bacterium.Separating the cell envelope compartments enables proteins to be localized to confirm where in the cell they function.Cell fractionation can also provide a first step jilungin dreaming tea in a protein purification strategy (Williams et al., 1998).This protocol has been designed to obtain the different fractions of P.

aeruginosa, namely the inner membrane, outer membrane, cytoplasmic and periplasmic compartments.Specific detection of the arginine specific autotransporter (AaaA) (Luckett et al., 2012) in the outer membrane of P.aeruginosa has been performed sara stedy stand aid using this protocol.